COMMENTARY ON MONTAGNIER
By Eleni Papadopulos-Eleopulos et
al.
Continuum Winter 1997
We would like to thank Djamel Tahi and Huw Christie for asking us
to comment on Professor Luc Montagnier's answers given in his
interview with Djamel Tahi. Before commenting we thought it would be
useful to start with a brief review of the methods used to prove the
existence of retroviruses, and Montagnier et al 1983 evidence for
the existence of "HIV".
Methods used to prove existence of retroviruses
It is generally accepted that Peyton Rous discovered retroviruses in
1911 when he induced malignancy in chickens by injections of cell-free
filtrates obtained from a muscle tumour. Similar experiments were
repeated by many researchers and the tumour inducing filtrates became
known as filterable agents, filterable viruses, Rous agents, Rous virus.
However, Rous himself expressed doubts that the agents which caused
tumours were infectious in nature. Indeed, Rous warned, "The first
tendency will be to regard the self-perpetuating agent active in this
sarcoma of the fowl as a minute parasitic organism. Analogy with several
infectious diseases of man and the lower animals, caused by
ultramicroscopic organisms, gives support to this view of the findings,
and at present work is being directed to its experimental verification.
But an agency of another sort is not out of the question. It is
conceivable that a chemical stimulant, elaborated by the neoplastic
cells, might cause the tumour in another host and bring about in
consequence a further production of the same stimulant".(1)
In 1928, AE Boycott, the President of the Royal Society of Medicine,
Section of Pathology, in his Presidential Address entitled "The
Transition from Live to Dead: the Nature of Filtrable Viruses", said:
"Another analogous phenomenon takes us, I think, a step further. The
products of autolysis of dead cells in the body, in suitable
concentration, stimulate tissue growth. It is a beautiful
self-regulating mechanism in which the amount of stimulus is
proportionate to the amount of cell destruction, and therefore to the
amount of cell growth required, and it is obviously of the highest
importance for survival - a far more potent factor in selection and
evolution than any disease has ever been. As it normally operates in
healing our cut fingers, the final result is simply the restoration of
the cells which were destroyed. But if the normal restraint exercised by
neighbouring tissues is evaded and use made of tissue cultures, the
products of autolysis or metabolism (in the form of extracts of tissues,
tumours, or embryos) stimulate growth indefinitely and a much larger
quantity of tissue may be obtained than we started with. From the
autolysis of this a larger amount of stimulating substance may be
obtained, and there seems no reason why this process of multiplication
should have any limit: normal tissues in the physical isolation of
tissue cultures are as immortal as malignant tissues in their
physiological isolation from the rest of the body...These products of
autolysis...have not received nearly as much attention as they deserve,
but they are probably of relatively simple and discoverable
constitutions. Yet applied to cells they cause growth, and in so doing
potentially increase their own quantity; this is very much what the Rous
agent does...As to its origin, all the evidence seems to concur in
indicating that the Rous virus arises de novo in each tumour.
There is no epidemiological evidence that cancer comes into the body
from outside; everything we know supports the classical view that it is
a local autochthonous disease. Experimental sarcomas produced by embryo
extract and indol, arsenic or tar have been transmitted by filtrates.
Epitheliomas are easily produced in mice by tar and in men by chronic
irritation; and if we believe that all malignant tumours contain more or
less of a carcinogenic agent akin to the Rous virus, it follows that we
can with a considerable degree of certainty stimulate normal tissues to
produce virus".(2)
Ten years earlier in an article entitled "The Plasmagene Theory of
the Origin of Cancer", Darlington, discussing the induction of cancer by
the Rous agent, the filtrable viruses and the "self-propagating"
particles transmitted by heredity but lying outside the nucleus found in
plants and "known as plasmagenes", wrote: "These infections, it will be
seen, are artificial, or at least unnatural. Now the distinction between
natural and artificial infection has long been known, although little
regarded, in the discussion of plant viruses. A number of aberrant
conditions can be transmitted from stock to scion, and some even have
arisen in a scion after it has been grafted on a healthy stock. These
are artificial diseases; they are not transmitted in nature, but only by
grafting. Some may have arisen by the mutation of self-propagating
proteins in the cells of plants propagated over long periods by
vegetative means (as tumours can be). Others have certainly arisen by
the migration or transplantation of proteins from one organism to
another. In either case they have a property of infection which they can
reveal only in artificial circumstances...We make a great mistake
therefore in calling them viruses; they are proviruses... One
more question is worth answering: What form would the mutant protein be
likely to take in the tumour cell? On account of its rapid
multiplication it might well show a higher degree of aggregation than
its progenitor. It would then appear as an alien particle in the mutant
cell. This is borne out by the electron microscope observations on two
chicken tumour agents of provirus type by Claude, Porter and Pickels
(1947)".(3)
The electron microscope observation by Claude et al is the
first report of virus-like particles in a tumour, the first electron
micrographs of the "Rous virus". Soon after many other researchers
reported these type of particles in many tumours, and as Boycott
predicted in "stimulated normal tissues". As far as Darlington's
prediction that these particles may be due to "a higher degree of
aggregation" of the cytoplasm it may be interesting to note that: (a)
for proteins, nucleic acids or protein/nucleic acid aggregation
(condensation, contraction) to take place, oxidation is necessary;(4)
(b) tumour tissues are oxidised;(4) (c) all the agents used to
"stimulate normal tissues" to induce retroviruses are oxidising
agents.(5-7)
In the 1940s, following the development of the electron microscope
(EM) and the technique of ultracentrifugation in density gradients, the
particles observed in malignant tissues could be isolated and thus
purified, that is, separated from everything else. Because these
particles were seen in malignant tissues "it has been judged that the
particles constitute the aetiological agent of the disease" and by the
1950's Rous's filtrable agents became known as oncoviruses (onkos =
tumour). The principal morphological characteristic of these particles
is a restricted range of diameters and the main physical characteristic
their density.(8) When the ultrastructure of these particles was
determined they were defined as particles with a diameter of 100-120nM
containing "condensed inner bodies (cores)" and surfaces "studded with
projections (spikes, knobs)".(9)
By the 1950s well-known retrovirologists such as JW Beard,
recognised that cells including uninfected cells, under various
conditions, were responsible for the generation of a heterogeneous array
of particles, some of which may look like oncoviruses. This "particle
problem" led to the opinion that to prove the existence of a retrovirus
"the scheme of approach, as well illustrated by that devised and
rigorously tested in investigations of viral agents, is relatively
simple. This consists in (1) isolation of the particles of interest; (2)
recovery (purification) of the particles in a given preparation that are
homogeneous with respect to particle kind; (3) identification of the
particles, and (4) analysis and characterisation of the particles for
the physical, chemical, or biological properties desired". Beard also
stressed that "identification, characterisation, and analysis are
subject to well-known disciplines established by intensive
investigations, and the possibilities have by no means been exhausted.
Strangely enough, it is in this field that the most frequent
shortcomings are seen. These are related at times to evasion of
disciplines or to their application to unsuitable materials. As was
foreseen, much of the interest in the more tedious aspects of particle
isolation and analysis has been diverted by the simpler and undoubtedly
informative processes of electron microscopy. While much can be learned
quickly with the instrument, it is nevertheless clear that the
results obtained with it can never replace, and all too often may
obscure, the need for the critical fundamental analyses that are
dependent on access to homogenous materials"(10) (italics ours).
Retrovirologists also agreed that "Virions of RTV (retroviruses )
have a characteristic buoyant density, and centrifugation to equilibrium
in density gradients is the preferred technique for purification of
RTV".11 At a European meeting on the use of centrifugation in density
gradients held at the Pasteur Institute in 1972 with Jean-Claude
Chermann as its secretary, it was stressed that once the culture fluids
(supernatants) are banded, the density band at which retroviruses are
trapped (this varies slightly with the substance used to manufacture the
gradients), must be thoroughly assayed. The assays consist of the
following:
"Assays for RNA Tumor Viruses
Physical
Electron Microscopy (neg stain and thin sect.)
Virus count
Morphology
Purity
Biochemical
Reverse transcriptase
60-70S RNA, total
RNA
Total protein
Gel analysis of viral and host proteins and
nucleic acids
Immunological
Gel diffusion
Complement fixation*
Immunofluoroescence*
Biological
Infectivity in vivo
Infectivity
in vitro
*With specific reagents for enveloped and internal antigens gs and
env".(12)
(Reverse transcriptase is an enzyme first discovered in oncoviruses
in 1970 (13) hence their present name retroviruses, and 60-70S RNA, the
"viral" RNA. Retroviruses are sometimes called RNA tumour viruses
because their genome consists of RNA and not DNA).
Thus the method specified at the Pasteur Institute in 1972 is no
different from that discussed by JW Beard two decades earlier. Indeed,
the method is basic logic applied to the definition of a virus. It is
impossible to claim that a protein or an RNA are retroviral unless it is
first proven these are constituents of a particle and that the particle
is infectious. As can be seen, the first step is electron microscopic
examination to prove that the band contains particles with the
morphological characteristics of retroviruses and, as Francoise
Barre-Sinoussi and Jean Claude Chermann pointed out at the Pasteur
meeting, that the band is pure, that is, it contains nothing else but
particles with "no apparent differences in physical
appearances".(14)
The second step in assaying the 1.16g/ml material is to prove that
the particles are able to reverse transcribe RNA into DNA. However, as
Gallo himself warned the finding of particles, even those containing
reverse transcriptase, is insufficient evidence to prove a particle is a
retrovirus. The complete proof depends on experiments to: (a) obtain
particles from a culture that are separate from everything else
(isolated) and show that the particles contain proteins and RNA but not
DNA and the proteins are coded by the RNA (the viral genome); (b) show
that when the particles are introduced into a culture of uninfected
cells, the particles enter the cells, the particles' RNA is reversed
transcribed into DNA which is incorporated into the cellular DNA; (c)
show that these cells in their turn produce retroviral-like particles;
(d) show that the particles produced by these cells contain proteins and
RNA which are identical with those of the original particles introduced
into the cells; (e) show that cell cultures identical to those in which
the retroviral-like particles were introduced do not produce such
particles when they are cultured in exactly the same conditions but
instead of the retroviral particles one introduces some other culture
material such as cellular microvesicles. This is because, unlike for any
other infectious agent, all cells contain retroviral genomes which under
appropriate conditions may be expressed in culture. That is, may lead to
the appearance of retroviruses known as endogenous retroviruses. It
follows that both the cells in the culture from which the original
particles were obtained as well as the culture into which they were
introduced may release identical retroviral particles even if the
particles that were introduced were not infectious. Therefore it is
absolutely imperative to have suitable controls.
Thus, to prove the existence of a retrovirus, one must isolate and
analyse the retroviral-like particles twice. The first time to obtain
and analyse the particle constituents released in the first culture. The
second time to prove that the particles released, if any, by the cell in
the second culture, are identical to the ancestral particles. The
crucial caveat in this procedure is the use of experimental techniques
to control for the effects of cocultivation, chemical agents and the
many other factors which themselves may induce retroviral phenomena
independent of exogenous retroviral infection.(15-17)
In conclusion, by the early 1980s, retrovirologists agreed that to
prove the existence of retroviruses one must first isolate (purify)
candidate particles and the method to achieve this was by banding in a
density gradient.
Summary of Montagnier and colleagues 1983 Science
paper
In 1983 Luc Montagnier and his colleagues from the Pasteur Institute
and other French researchers published a paper which is considered the
first study in which the existence of "HIV" was proven. The paper is
entitled "Isolation of a T-Lymphotropic Retrovirus from a patient at
risk for Acquired Immune Deficiency Syndrome (AIDS)"(18) with Francoise
Barre-Sinoussi as principle and Jean Claude Chermann as second author.
The authors' claim to have isolated a retrovirus and thus proven its
existence was based on the following experiments:
1. Lymphocytes from the lymph nodes of two patients with
lymphadenopathies as well as peripheral blood mononuclear cells from
these patients "were put in culture medium with phytohemagglutinin
(PHA), T-cell growth factor (TCGF), and antiserum to human a
interferon...In the mouse system, we had previously shown that antiserum
to interferon could increase retrovirus production by a factor of 10 to
50". The supernatants were regularly assayed for reverse transcriptase
activity (RT) using the synthetic template primer An.dT12-18. "After 15
days of culture, a reverse transcriptase activity was detected in the
culture supernatant of the lymph node culture" of one of the patients,
the first patient. (The level of activity is not given). "Peripheral
blood lymphocytes cultured in the same way were consistently negative
for reverse transcriptase activity even after 6 weeks". So were both
cultures from the second patient. Apparently the detection of RT
activity was considered evidence for infection with a retrovirus.
2. Lymphocytes from an adult healthy blood donor were cultured
(culture conditions not given) and after three days half the culture was
cocultured with lymphocytes from the patient's culture in whose RT was
detected. (Conditions not given). "Reverse transcriptase activity could
be detected in the supernatant on day 15 of the cocultures", (level of
activity not given) but not in the culture of the blood donor. (It is
not mentioned if the conditions in the blood donor culture were the same
as the coculture conditions. However, it is obvious that the blood donor
cells were not cocultured with lymphocytes from lymph nodes of patients
who were not at risk of AIDS but who otherwise had similar clinical and
laboratory abnormalities as patient number one. Given that
co-cultivation leads to the appearance of endogenous retroviruses this
is a significant omission from the experimental protocol).
3. Normal umbilical cord lymphocytes were cultured for three days
(culture conditions not given), after which supernatants from the
coculture and polybrene were added. "After a lag period of 7 days, a
relatively high titer of reverse transcriptase activity was detected".
(In fact the activity was relatively low, no more than 8,000 counts/min.
Background activity as high as 4000 counts/min have been reported.(19)
"Identical cultures" to which supernatant has not been added remained
negative. (Since no supernatant was added the cultures could not have
been identical. Since supernatant from non-infected cultures added to
normal non-infected cells leads to the appearance of endogenous
retroviruses this is also a significant difference). Commenting on the
findings in the three experiments the authors wrote: "These two
successive infections clearly show that the virus could be propagated on
normal lymphocytes from either newborns or adults". The data from the
three experiments apparently were also considered proof of "isolation",
however, "That this new isolate was a retrovirus was further indicated
by its density in a sucrose gradient, which was 1.16".
4. The evidence from the sucrose gradients consisted of two parts.
(a) the supernatant from the cord blood lymphocytes in which RT activity
was detected was banded in sucrose density gradients. Maximum RT
activity was reported at the 1.16g/ml band. (b) to the cord blood
lymphocyte culture in which RT activity was detected [35S] methionine
was added, that is radioactive methionine, an amino acid which is
incorporated into growing protein chains and whose radioactivity allows
detection of such proteins. Two types of experiments were performed with
this culture, one with the cells and the other with the supernatant: (i)
a cell extract was lysed (broken apart) and centrifuged. To parts of the
cellular supernatant various sera (containing antibodies) were added and
the proteins were electrophoresed (separated using an electric field) on
a polyacrylamide-SDS slab gel. Many proteins were found to react, not
only with the sera from the two patients with multiple lymphadenopathies
but also with sera from a healthy donor and a normal goat. (ii) the
culture supernatant was banded in a sucrose density gradient. Although
no mention is made of EM studies of the 1.16g/ml band, it was claimed
that the band represented "purified, labelled virus from patient 1". The
1.16g/ml band was reacted with the sera of the two patients as well as
two healthy blood donors and was processed in the same way as the
cellular extract. Although in the published manuscripts it is virtually
impossible to distinguish proteins reacting with any sera, even with the
sera from the two patients, in the text it is stated that "when
purified, labelled virus [the 1.16g/ml band] was analysed [reacted
with the sera] three major proteins could be seen: the p25 protein and
proteins with molecular weights of 80.000 and 45.000. The 45K protein
may be due to contamination of the virus by cellular actin which was
present in imunoprecipitations of all cell extracts." (italics ours) EM
studies of the cord blood lymphocytes culture "showed characteristic
immature particles with dense crescent (C-type) budding at the plasma
membrane...The virus is a typical type-C RNA tumour virus".
Comments on answers Montagnier
A1. 1. If "culture, purification of the material by
Ultracentrifugation, Electron Microscopic (EM) photographs of the
material which bands at the retrovirus density, characterisation of
these particles, proof of the infectivity of the particles" is not
isolation, then why did Montagnier and his colleagues claim in 1983 to
have isolated "HIV" by either performing or claiming to have performed
all but one (no EM photographs of the banded material) of these
procedures? Why in the 1984 paper where they claimed the first isolation
of "HIV" from haemophiliacs, as well as in their other studies that year
in which they also claim "HIV" isolation they have either performed or
claimed to have performed all but one of these steps?(20-21) Why in
their study entitled "Characterisation of the RNA depended DNA
Polymerase of a new human T lymphotropic retrovirus (lymphadenopathy
associated virus)"(22) did they state that the virus was "purified on
sucrose gradient using isopycnic centrifugation (8)"? Reference 8 is the
paper presented by Sinoussi and Chermann at the 1972 Pasteur Symposium
where they stressed the importance of showing that the banded material
contained nothing else but particles with "no apparent differences in
physical appearances".(14)
2. The finding of some or all of the phenomena Montagnier outlines
are not proof of isolation. These phenomena can be considered only proof
for viral detection and then, if and only if, they are specific to
retroviruses. The word "isolation" is derived from Latin "insulatus"
meaning "made into an island". It refers to the act of separating an
object from all the extraneous matter that is not that object. Here the
object of interest is a retroviral particle. The words 'isolation' and
'passing' have different and distinct meanings. 'Isolation' means to
obtain an object, a retrovirus particle for example, separate from
everything else. 'Passing' means to transfer an object (which may or may
not be isolated) from one place to another, for example, from one
culture to another. Therefore, even if one assumes that the "something"
which Montagnier and his colleagues passed from one culture to another
by means of transferring cells or culture supernatants was a retrovirus,
and that it was passed to an infinite number of successive cultures, it
still is not evidence for isolation. For example, if one has a series of
bottles containing water in which the first has a dye added, then takes
part of the first and puts it in the second, and from the second passes
a sample into the third et cetera, clearly this procedure has not
isolated the dye from the water. A culture contains a myriad of things
and thus by definition is not evidence for isolation of an object. The
only way possible to claim that one has "made a culture of the virus",
is to have had proof for the existence of the virus before making a
culture. The only thing which Montagnier and his colleagues have proven
is the emergence in the coculture with "lymphocytes from a blood donor"
of RT activity. Detection of an enzyme in a culture, even if specific to
retroviruses is not evidence for isolation. For example, the measurement
of cardiac or liver enzymes in cases of myocardial infarction or
hepatitis respectively cannot be construed as "isolation" of the heart
or liver. The finding in the culture of particles with the morphological
characteristics of retrovirus and of reverse transcriptase activity
either in the culture or the 1.16g/ml band, even if "truly specific of
retroviruses" is not evidence for retroviral isolation. Even if
Montagnier and his colleagues knew beforehand that some of the proteins
present in the culture or the 1.16g/ml band were retroviral, and the
patients had retroviral antibodies which reacted with these proteins,
such a reaction is not evidence for isolation. Argument based on
analogies, or even on knowledge of other retroviruses, cannot be
construed evidence for isolation. For example, observing something in
the ocean which looks like a fish (even if it is a fish), is not
equivalent to having the fish in your frypan separate from everything
else that occurs in the ocean.
3. We agree with Gallo that Montagnier et al did not present
proof for "true isolation" of a retrovirus, any retrovirus, either old
or new, exogenous or endogenous.
4. The "knowledge of other retroviruses" shows that not all particles
with RT activity and "visual properties of retrovirus" are viruses. This
is a fact acknowledged even by Gallo well before the AIDS era.(23) It
also shows that RT is not "truly specific of retroviruses". Non-infected
cells as well as bacteria or viruses other than retroviruses have RT.
According to some of the best known retrovirologists including its
discoverers, as well as Nobel Laureate and Director of the US National
Institutes of Health, Harold Varmus, reverse transcriptases are present
in all cells including bacteria.(13,24-25) Indeed RT activity has been
reported in many of the cell lines from which "HIV" is "isolated",
including H9 and CEM as well as normal lymphocytes even when they are
not infected with "HIV".(26-27) Montagnier, Barre-Sinoussi and Chermann
themselves have shown that RT activity is not specific to retroviruses.
In their 1972 paper Barre-Sinoussi and Chermann wrote: "There was
significant activity in the sample zone and the fastest sedimenting
peak, consisting mainly of cell debris. This enzymatic activity can be
explained by the presence of some virus particles in these regions, and,
since similar polymerase activity has been found in normal cells, may be
mainly ascribed to the cellular enzyme". In this interview, Luc
Montagnier answering question 14 says: "For example, one day I had a
very fine peak of RT, which F Barre-Sinoussi gave me, with a density a
little bit higher, 1.19 and I checked! It was a mycoplasma, not a
retrovirus". How is it then possible for Montagnier to say that RT is
specific to retroviruses? We agree that RT activity is characteristic of
retrovirus. However, 'specificity' does not have the same meaning as
'characteristic'. Hair is characteristic of human beings but not every
animal with hair is human.
5. Isolation means to obtain an object separate from everything else.
Retroviruses are particles and no amount of "analogy" can prove that one
has isolated a retroviral particle. "Knowledge of other retroviruses"
can be of assistance in choosing the best method to obtain isolation.
The "knowledge of other retroviruses" shows that the best, but by no
means perfect method to isolate and prove the existence of retrovirus,
is to perform isopycnic (identical density of particle and portion of
the gradient) banding and to perform all the assays specified at the
1972 Pasteur symposium. The "knowledge of other retrovirus" also shows
that there is nothing specific about the morphology of retroviral
particles, protein-antibody reactions or even banding at the density of
1.16g/ml in sucrose density gradients. Retroviral particles band at the
density of 1.16g/ml but not everything at that density, including
particles with the morphology of retroviral particles, is a
retrovirus.(11-13,28) To remind ourselves this is the case, one needs go
no further than to consider the "first" human retrovirus, "HL23V".
In the mid 1970s Gallo and his colleagues reported the isolation of
the first human retrovirus. In fact the evidence for the isolation of
"HL23V" surpassed Montagnier's et al and everybody else's
evidence for "HIV" in at least three important aspects. Unlike "HIV", in
the case of "HL23V" Gallo's group: (a) reported the detection of RT
activity in fresh, uncultured leucocytes; (b) did not need to stimulate
their cell cultures with various agents. (Both Montagnier and Gallo
concede that none of the phenomena which they say prove the existence of
"HIV" can be detected unless the cultures are stimulated with several
agents); (c) published an electron micrograph of virus-like particles
banding at a sucrose density of 1.16g/ml.(23-29) However, today nobody,
not even Gallo, considers "HL23V" as being the first human retrovirus or
even a retrovirus. (For a more detailed discussion see
Papadopulos-Eleopulos et al (30-32)). One also must not forget
the following additional knowledge in relation to retroviruses: (a) the
lesson of the enzyme adenosine triphosphatase. Like RT, this enzyme was
considered to be specific to retroviruses and at least in the 1950s was
used not only for their detection and characterisation but also for
their quantification.(8-11) Yet at present it is accepted that this is
one of the most widely spread enzymes. (b) a much higher percentage of
sera from AIDS patients and those at risk reacts with proteins of
endogenous retroviruses than the sera of healthy people, 70% versus
3%.(33)
A2. 1. It is true that Montagnier and his colleagues found a
peak of RT activity at the density of 1.16g/ml. However, finding this
peak is not proof that the band was made up of retrovirus particles
either pure or impure. Therefore this evidence cannot be considered that
"one has fulfilled this criterion for purification".
2. In the same issue of Science where Montagnier and his
colleagues published their study Gallo pointed out that "the viral
envelope which is required for infectivity is very fragile, it tends to
come off when the virus buds from infected cells, thus rendering the
particles incapable of infecting new cells". Because of this Gallo
claimed that "cell-to-cell contact may be required for retroviral
infection".(34) At present all "HIV" experts agree that for "HIV"
infectivity gp120 is absolutely necessary. In 1993 Montagnier himself
said that for the "HIV" particles to be infectious they must first bind
to the cellular CD4 receptor and that "The gp120 is responsible for
binding the CD4 receptor".(35-36) However, to date nobody has published
EM of cell-free particles having the dimension of retroviral particles
and also knobs, spikes, that is gp120, not even Hans Gelderblom and his
colleagues from the Koch Institute in Berlin who have conducted the most
detailed electron microscopy studies of the particles present in
culture/cocultures containing tissues derived from AIDS patients. In one
of their latest publications where this matter is discussed they
estimate that immediately after being released, "HIV particles" possess
an average of 0.5 knobs per particle but also pointed out that "it was
possible that structures resembling knobs might be observed even when
there was no gp120 present, i.e., false positives".(37)
This means that neither Montagnier and his colleagues nor anybody
else subsequently could infect the cultures with cells from healthy
donors, umbilical cord lymphocytes or any other cultures with the
"purified HIV" or, even the cell-free fluids (the culture supernatant)
even if the "purified" virus contained nothing else but particles. In
other words, it is impossible for Montagnier and his colleagues to have
had any infectivity even "a little" with either the culture supernatant
or the "purified labelled virus". For the same reason the "second
strain" could not be contaminated by "the first". Furthermore, since
Montagnier et al provided Gallo with cell-free supernatants, it
would have been impossible for the Gallo cultures to be contaminated
with BRU, LAI or a mixture.
3. Montagnier's virus did not come "from an asymptomatic patient" but
a patient with "lymphadenopathy and asthenia". Neither in their study
nor even today, after nearly fifteen years of "HIV", is there proof for
the existence of a human retrovirus which has the ability to "kill
cells". The study which at present is most often quoted as proving "HIV"
kills T4 cells, considered to be the "hallmark" of AIDS, was published
in 1984 by Montagnier and his colleagues. They cultured CD4+ (T4) cells
from a haemophilic patient who was "an asymptomatic virus carrier", "in
the presence of phytohemagglutinin (PHA) followed by IL-2". In the
culture they detected RT activity and "virus particles characterised by
a small eccentric core". The number of T4 (CD4+) cells in the culture
were measured by counting the number of cells able to bind a monoclonal
antibody claimed specific for the CD4 protein. The number of cells which
were able to do so decreased with time. Discussing their finding they
wrote, "This intriguing phenomenon may be due to virus-induced
modulation at the cell membrane, or by steric hindrance of the antibody
binding site", that is, the decrease is not due to cell killing.(38-39)
Given their data, the conclusion that the decrease in T4 cells is not
due to cell killing is not surprising. However, their conclusion that
the effect may be induced by the "virus", is surprising. Montagnier and
his colleagues were aware of the experimental evidence which showed that
under certain conditions, (including exposure to PHA, IL-2 and other
oxidising agents) decrease in T4 cells appears in the absence of HIV. In
this type of culture, T-cells lose their CD4 marker and acquire other
markers, including CD8, while the total number of T-cells remains
constant.(40-43) Furthermore, they had evidence that in "infected cells,
this phenomenon cannot be detected unless the culture is stimulated by
substances such as PHA or antigens. (Proteins such as the "non-HIV"
proteins present in the "infected" cultures (39)) Given the above facts
it is even more surprising that Montagnier and his colleagues did not
have controls, that is, cultures of T4 cells originating from patients
who were not at risk of AIDS but who nonetheless were sick and to which
they added PHA and IL-2. Such experiments were reported in 1986 by Gallo
and his colleagues. They presented data on three cell cultures which
contained 34% CD4 cells to begin with: One culture was "infected" and
stimulated with PHA, the other was not infected but was stimulated with
PHA and the third was neither infected nor stimulated. After two days of
culture, the proportion of CD4+ cells in the stimulated-uninfected and
stimulated-infected culture was 30% and 28% respectively, while at 6
days the number was 10% and 3%. The number of CD4+ cells did not change
significantly in the non-infected non-stimulated culture.(44)
By 1991 Montagnier and his colleagues had performed experiments with
uninfected, unstimulated cells when they studied "HIV" induced
apoptosis, which was said (and is still said by many), to be the
principle mechanism by which "HIV" kills cells. They showed that in
acutely "HIV infected" CEM cell cultures in the presence of mycoplasma
removal agent, cell death (apoptosis) is maximum at 6-7 days post
infection, "whereas maximal virus production occurred at Days 10-17",
that is, maximum effect preceded the maximum cause. In chronically
"infected" CEM cells and the monocytic cell line U937, no apoptosis was
detected although "these cells produced continuously infectious virus".
In CD4 lymphocytes isolated from a normal donor, stimulated with PHA and
"infected with HIV" in the presence of IL-2, apoptosis becomes
detectable 3 days post infection and clearly apparent at 4 days.
"Intriguingly, on the 5th day" apoptosis became detectable in
"uninfected", PHA stimulated cells. They concluded: "These results
demonstrate that HIV infection of peripheral blood mononuclear cells
leads to apoptosis, a mechanism which might occur also in the absence of
infection due to mitogen treatment of these cells".(45) In conclusion,
all the presently available data shows that "HIV infection" in the
absence of stimulating agents neither decrease the T4 cell number, nor
induce apoptosis, while stimulating agents (similar to the those
patients at risk of developing AIDS are exposed) do so in the absence of
"HIV". That is, neither the "HIV", which Montagnier and his colleagues
"stumbled" at the beginning, nor any other "HIV" since then has been
shown to "kill cells".
A3. Retrovirus are not esoteric, nuclear or cosmological
notions whose postulated existence can only be inferred by indirect
observations. They are particles which can be seen, albeit not with the
naked eye. Since Montagnier and his colleagues admit to not seeing
particles at the 1.16g/ml band having the morphology of retrovirus, to
claim the presence of a retrovirus much less a "purified virus" is
totally unsubstantiated and defies belief. The 1.16g/ml band can be
likened to a fishing net. The difference is that the band traps objects
according to their density, not their size. Imagine a fisherman who sees
in the ocean many different objects some of which may be fish. He throws
the net, waits, and upon retrieval of the net performs a thorough
examination of its contents and shows that it contains many sea
creatures but nothing that looks like a fish. Yet strange as it may
seem, he claims to have caught a fish. In fact, he claims that the net
has nothing else but pure fish.
A4. Although budding from the cell membrane is the manner in
which retroviral particles appear, this process is not virus specific.
In other words, just because a particle buds and has the morphological
characteristics of retroviral particles does not prove it is a
retrovirus. That this is the case can be illustrated by two facts and by
quoting two of the best known retrovirologists: "Budding virus-like
particles" have been found in non-infected "T-cell lines CEM, H9 and
C8166; In 2 lines of EBV transformed B-cell lines; and in cultures of
primary human lymphoid cells from cord blood, which were either PHA
stimulated or not and grown with or without serum and in cord
lymphocytes directly after Ficol separation"(46) (italics ours).
Following an extensive, in vivo study conducted by O'Hara and
colleagues from Harvard, "HIV particles" were found in 18/20 (90%) of
patients with enlarged lymph nodes attributed to AIDS. However,
identical particles were also found in 13/15 (87%) of patients with
enlarged lymph nodes not attributed to AIDS and at no risk for
developing AIDS. These data led the authors to conclude, "The presence
of such particles does not, by themselves indicate infection with
HIV".(47)
In 1986 Gallo and his colleagues discussing the "First isolation of
HTLV-III" wrote: "At the time we obtained LAV it was the contention of
several experts in virus morphology that the particles shown in the
electron micrograph published in Science by Barre-Sinoussi et al
was an arena virus...Since we considered the mere detection of virus
particles in cultures from AIDS and ARC patients to be insufficient to
confirm scientifically our hypothesis that such particles were
implicated in the aetiology of the disease, we decided first to obtain
specific reagents against the new virus in order to publish definite
results concerning AIDS aetiology".(48) According to Peter Duesberg the
"HIV" "particles and proteins could reflect non-viral material
altogether".(49)
A5. In their study Montagnier and his colleagues wrote:
"Electron microscopy of the infected umbilical cord lymphocytes showed
characteristic immature particles with dense crescent (C-type) budding
at the plasma membrane...This virus is a typical type-C RNA tumor
virus". In 1984 Montagnier, Barre-Sinoussi and Chermann reported that
their virus was "morphologically similar to D particles such as those
found in Mason-Pfizer virus or the virus recently isolated from simian
AIDS".(38) (By 1984 researchers from the primate research centres in the
United States claimed the existence of AIDS in monkeys and that the
cause of AIDS was a type-D retrovirus similar to the Mason-Pfizer virus,
a typical type-D retrovirus and suggested that the monkey AIDS and these
retroviruses could be helpful in the study of human AIDS and "HIV").
In the same year, in yet another publication, Montagnier et al
claimed that the "HIV" particles had "morphology similar to that of
equine infectious anaemia virus (EIAV), and D type particles". The EIAV
and the visna virus are neither type C nor type D retroviruses but
lentiviruses, that is, viruses which have totally different morphology
and said to induce diseases long after infection. (By the time this
paper was published it was realised that patients who had a positive
"HIV" antibody test did not develop AIDS immediately, that is, there was
a delay between the positive test and the appearance of AIDS.) It is
most astonishing that the morphology of one and the same virus is able
to change genus from a typical type-C to a typical type-D particle and
then to a completely different subfamily, namely a typical lentivirus,
apparently at will. (The family Retroviridae is divided in three
subfamilies, Oncovirinae, Lentivirinae and Spumavirinae. Oncovirinae are
in turn divided into genus type-B,-C and -D particles. These findings
are analogous to describing a new species of mammal as human, a gorilla
and an orang-utan).
A6. 1. Apart from retroviruses other particles may possess
"the assemblage of properties" (the density, RT, budding and the analogy
with the visna virus). It follows that the detection of particles having
this "assemblage of properties" is not proof that the detected particles
are retroviruses. In fact, Montagnier and his colleagues did not report
the detection of "HIV" particles having this "assembly of properties".
Since Montagnier and his colleagues could not find particles with the
morphological characteristics of retrovirus at the "density" of 1.16
gm/ml, even after "a Roman effort", it follows that the evidence for the
existence of "HIV" from the density gradient was not only non-specific
but was non-existent. (This fact alone is sufficient to dismiss any
claim of proof for the existence of a retrovirus, no matter what else
they found anywhere including budding particles from the cell surface,
retrovirus-like particles in the culture, RT at the "density" or
proteins at the same density which react with patient sera). 2. It is
true that Montagnier et al reported RT activity at the density of
1.16g/ml but since: (a) Barre-Sinoussi and Chermann accept that cells
and cellular fragments also have RT activity; (b) at the 1.16g/ml band
no particles with the morphological characteristics of retrovirus were
seen; (c) at that density Montagnier et al found cellular
fragments, it follows that the evidence for the existence of "HIV" by
detecting RT activity at that density was not only not specific but
non-existent. Given the facts that: (a) there are significant
differences in the nature of the budding processes between type-C,
type-D particles and lentiviruses50 and that in 1983 Montagnier et
al reported their retrovirus as type-C and in 1984 as either type-C
or type-D, and even later that year as EIAV; (b) visna virus and EIAV
are lentiviruses, it follows that at least up mid 1984 Montagnier's
et al evidence for the existence of "HIV" (if "HIV" is a
lentivirus) from "pictures of budding" and the analogy with EIAV and
visna virus was not only non specific but non-existent.
A7. We agree there are endogenous retroviruses (but it is of
interest that up until 1994 "there are no known human endogenous
retroviruses"(51)). These endogenous retroviruses cannot be
distinguished from exogenous retroviruses either morphologically or
chemically. Furthermore, evidence exists which shows that 70% of AIDS
patients and those at risk compared with 3% of people not at risk have
antibodies to endogenous retroviruses.(33) Given these facts and the
culture conditions which Montagnier and his colleagues and all other
"HIV" researchers use to detect "HIV" together with the presently
available data on "HIV" and AIDS, it is more probable that "HIV" (if
proven to exist) is an endogenous retrovirus rather than an exogenous
retrovirus.
Part of the data related to the culture conditions can be summarised
as follows: In culture, cells sooner or later start to release
endogenous retrovirus. The appearance of endogenous retrovirus can be
accelerated and the yield increased up to a million fold by stimulating
the culture with mitogens, co-cultivation or by adding to the culture
supernatant from normal, unstimulated cell cultures. Indeed, as far back
as 1976 retrovirologists recognised that "the failure to isolate
endogenous viruses from certain species may reflect the limitation of
in vitro cocultivation techniques".(52) To detect the
"assemblage" of the "four characteristics" of "HIV", Montagnier et al
(as well as everybody else) employed at least two of the above
techniques. In fact, both Montagnier and Gallo admit that not one of the
four "characteristics" can be detected unless the cultures are
stimulated. Similarly, part of the data related to "HIV" and AIDS can be
summarised as follows: (a) It is true that endogenous retroviruses may
have no pathological role in AIDS, but it is also true that to date
neither is there such proof for "HIV".(53) According to Montagnier and
Gallo the "hallmark" of immunodeficiency in AIDS is the decrease in T4
cells, said to be the result of killing of T4 by "HIV". However
Montagnier and his colleagues admit as far back as 1984 that at least in
vitro the observed decrease in T4 cells after "HIV" infection is not due
to cell killing but decreased binding of the T4 (CD4) antibody to the
cells. Two years later the Gallo team's experiments proved beyond doubt
that the decrease in T4 cells (of the CD4 antibody binding) was not due
to "HIV" infection but to the PHA which was present in the "HIV"
preparation. As mentioned, at the beginning of the AIDS era there was
ample evidence that treatment of cell cultures with PHA and other
oxidising agents leads to decreased binding of the CD4 antibody and to
increase binding of the CD8 antibody, that is, a decrease in T4 cells
was accompanied by increase in T8 cells, while the total cell number
remained constant. AIDS patients and individuals belonging to the AIDS
risk groups are continuously exposed to strong oxidising agents. At
present it is accepted that in both AIDS patients and those at risk, the
decrease in T4 cells is accompanied by an increase in T8, while the T4 +
T8 cell number remains constant.(53) Also, it is of interest to note
that as far back as 1985 Montagnier wrote: "This syndrome [AIDS] occurs
in a minority of infected persons, who generally have in common a past
of antigenic stimulation and of immune depression before LAV
infection"(54), that is, Montagnier recognised that in the AIDS risk
group, immune deficiency precedes "HIV" infection. In 1984 Montagnier
and his colleagues including Barre-Sinoussi and Chermann stated that
"Definite evidence will require an animal model in which such viruses
[LAV, HTLV-III=HIV] could induce a disease similar to AIDS." Up to
today, no such model exists. Nonetheless, when pursued by the Nobel
Laureate Kary Mullis for even one scientific paper proving the HIV
theory of AIDS, Montagnier advised him "Why don't you quote the work on
SIV" (Simian immunodeficiency virus);(55)
(b) Unlike endogenous retroviruses which are transmitted vertically,
"HIV" is said to be transmitted horizontally especially by sexual
intercourse. Indeed at present it is generally accepted that the vast
majority of individuals have been infected via heterosexual contact.
According to Montagnier and Gallo the first study to have proven beyond
doubt that "HIV" is a bidirectionally heterosexually transmitted virus
was published in 1985 by Redfield et al. However, in a book
published in 1990 entitled AIDS and Sex, its editors, Bruce
Voeller, June Machover Reinisch and Michael Gottlieb, discussing this
cross-sectional study, as well as other similar studies, wrote:
"government researchers published data indicating that United States
armed forces personnel infected with HIV-1 had caught the virus from
prostitutes, triggering calls for increasing campaigns against
prostitution. When infected soldiers were interviewed by nonmilitary
researchers whom they trusted, it became clear that nearly all had been
infected through intravenous drug use or homosexual contact, acts for
which they could be expelled from the armed services, which prevented
them from being candid with the original military researchers. In each
of these flawed published studies, researchers, journal editors, and
peer reviewers failed to correct mistakes that should have been
recognised".
Nancy Padian from the Department of Epidemiology and Biostatistics,
University of California, and her colleagues, who to date have conducted
the most thorough studies on heterosexual transmission discussing
Redfield's et al study as well as other studies who claimed proof
of such transmissions, wrote in 1991: These "studies may not have
adequately controlled for other confounding nonsexual routes of
transmission such as risks associated with intravenous drug use. At
first blush, cases that appear attributed to heterosexual transmission
may, after in-depth interviewing, actually be linked to other sources of
risk...because partner studies are by definition not random samples, and
most reported results are based on retrospective or cross-sectional
analyses, some studies may overselect couples in which both partners in
a couple are infected because such couples may be more easily
identified, thus biasing transmission rates. Furthermore, it is often
difficult to establish the source of infection in such couples. When few
prospective data are available, enrolling monogamous couples in which
the serostatus of the partner is unknown, as was the case for most
couples in this study, is one of the only ways to control for this
bias".(56) Indeed, there is no proof from the prospective studies, few
as they are, that "HIV" is sexually transmitted.(57-58)
In her ten year study, unquestionably the longest and the best study
of its kind, Padian (59) and her colleagues have spared no effort in an
attempt to prove that "HIV" is heterosexually transmitted. There were
two parts in her study, one cross-sectional, the other prospective. In
the former, of 360 female partners of infected male index cases, "The
constant per-contact infectivity for male-to-female transmission was
estimated to be 0.0009". The risk factors for seroconversion were: (i)
anal intercourse. (Montagnier himself showed that a positive antibody
test reverts to negative and a low T4 cell count to normal by stopping
anal intercourse, which means that the positive outcome is not due to a
retrovirus;(60) (ii) having partners who acquired this infection through
drug use (Padian herself says that this means that the women may also be
IV users); (iii) the presence in the female of STDs. (antibodies to
their causative agents may cross-react with the "HIV" proteins;(31) Of
82 negative male partners of positive female index cases only two
seroconverted. They estimated that the likelihood of female-to-male
transmission was 8 times lower than for male-to-female. Padian herself
questioned the validity of these two cases. For the first one she gave
several reasons in 1991, when this case was reported for the first time.
In the second case they mentioned the fact that "chlamydia was
transmitted simultaneously or close to transmission of HIV is striking",
that is, the positive "HIV" antibody test appeared at the time when he
became infected with chalmydia.
In the prospective study, starting in 1990, "We followed 175
HIV-discordant couples over time, for a total of approximately 282
couple-years of follow-up...The longest duration of follow-up was 12
visits (6 years). We observed no seroconversions after entry into the
study...At last follow-up, couples were much more likely to be abstinent
or to use condoms constantly ...Nevertheless only 75% reported
consistent condom use in the 6 months prior to their final follow-up
visit". Note: Not only seroconversion were reported only in the
cross-sectional study but all the cases were diagnosed before 1990.
However: (i) All the "HIV" experts agree that the specificity of the
test kits used then was inferior to those used at present; (ii) The WB
criteria used to define "infection" then are not sufficient at present.
Even if one accepts Padian et al data from the crossectional study, they
have estimated the risk to a non-infected male of acquiring "HIV"
infection from his infected female partner per contact is 0.00011
(1/9000). This means that on average, males having sexual intercourse
daily with an infected female partner for sixteen years (that is, 6000
contacts at 365 per year), would score a 50% probability of becoming
infected. If sexual intercourse takes place on average weekly then it
would take one hundred and fifteen years to reach the same probability.
Under such circumstances one must question how "HIV" could become
epidemic as the result of bi-directional heterosexual transmission.
A8. 1. In the Montagnier et al 1983 study, the
detection of nothing else but RT activity in the stimulated cultures of
lymphocytes originating from a gay man was considered proof that he was
infected with a retrovirus. The finding of the same activity in the
supernatant of a co-culture of the same cells with lymphocytes from a
healthy blood donor was considered proof of passing the retrovirus from
the gay man's lymphocytes to the donor's lymphocytes and also for virus
isolation. However, passing an activity (RT) is not the same as passing
an object (retrovirus).
Furthermore, since non "HIV" infected lymphocytes as well as many
bacteria and viruses other than retrovirus possess RT activity (RT
activity has been reported in many non-"HIV" infected cell lines used to
isolate HIV such as H9 and CEM and as far back as 1972 in normal, PHA
stimulated lymphocytes), finding RT activity in successive lymphocyte
cultures each of which contains material which originated from the
preceding one, is not proof even for passing RT activity. To illustrate
what Montagnier and his colleagues have done, let us return to the
analogy of the fisherman and his net: Assume the fisherman casts his net
and catches some sea creatures. He leaves a few in the net as bait and
then throws it out again. This time, in addition to sea creatures he
catches some fish as well. He removes the fish, leaves some sea
creatures in the net, throws the net again and this time he catches even
more fish. He repeats the procedure several times and every time he
catches more fish. Like Montagnier et al who remove the cells and
re-use the supernatants, the fisherman removes the fish and re-uses the
sea creatures ("the bait"). Clearly the fish caught in the net are not
offspring of the "bait". The purpose of the "bait" is to create the
right conditions for fish to appear in the net. (Indeed, real fisherman
spend a lifetime determining the right conditions). All the fisherman is
"passing" is the means for catching the fish, not the fish themselves.
Similarly, Montagnier et al appear to be "passing" the conditions
to generate RT activity thus generating the illusion of "passing" RT
activity.
2. Having a peak of RT activity is not proof for having "replication"
of a retrovirus. Keeping track of RT is not the same thing as keeping
"track of the virus".
3. Let us assume that one has isolated and proven the existence of a
retrovirus in cultures with tissues originating from humans. "The first
question put" by Nature is: 'Is it an endogenous retrovirus?'
Only when one has evidence that it is neither an exogenous nor an
endogenous human retrovirus does the question of "laboratory
contamination" with animal retroviruses arise.
4. What the patient had was antibodies which reacted with a protein
which in sucrose density gradients banded at 1.16g/ml. Since at that
density Montagnier and his colleagues could not find particles with the
morphological characteristics of a retrovirus, the evidence that this
protein was retroviral was non-existent. In fact they had no evidence
that the protein was embodied even in non-retroviral particles, any
particles whatsoever present at that density.
5. If Montagnier and his colleagues somehow knew beforehand that the
protein which banded at 1.16g/ml and reacted with the gay man's serum
was the protein of a retrovirus which was present in his lymphocytes
(and not the lymphocytes of the healthy donor or the umbilical cord),
and at the same time that the antibodies were directed against "his own
virus", why was it necessary to have all these experiments to prove its
existence?
A9. Even though they had RT activity, at the density of
1.16g/ml they had no evidence for the existence of retroviral particles
and thus the activity could not be considered proof for the existence of
such particles.
A10. In 1983, Montagnier, Barre-Sinoussi and Chermann and
their colleagues proved the existence of the enzyme reverse
transcriptase "using the ionic conditions described for HTLV-I", that
is, "5mM Mg2+" and "poly(A).oligo-(dT)12-18 as template primer". These
conditions and this template primer may be characteristics for
retroviruses but they are not specific for retroviral RT nor indeed any
RT. Even before the AIDS era it was known that this template-primer,
under the conditions used by Barre-Sinoussi, Montagnier and their
colleagues, can be transcribed not only by RT but by cellular DNA
polymerases as well. Suffice to mention the study entitled:
"Characteristics of the RNA dependent DNA polymerase [RT] of a new human
T lymphotropic retrovirus (lymphadenopathy associated virus)" ("HIV") in
which Montagnier, Barre-Sinoussi, Chermann and their colleagues
"characterised" the "HIV" RT. There they used the same ionic conditions
as in 1983 and three template primers "Activated DNA", poly
(A).oligo-(dT)12-18 and poly Cm .oligo-dG 12-18. They reported that
while poly Cm .oligo-dG 12-18, "a reverse transcriptase specific
template primer" was transcribed only by the "HIV infected" cells,
"Activated DNA" and poly (A).oligo-(dT)12-18 were transcribed by both
infected and non-infected cells.22 In other words, finding RT activity
by using the template primer An.dT12-18 is not even proof for the
existence of RT and even less for the existence of a retroviral RT.
A11. No comment.
A12. No comment.
A13. We agree with Montagnier that when using lymphocyte
cultures infected with exogenous retroviruses such as MT2, MT4 and H9
(HUT-78), all of which originated from patients with "adult T4-cell
leukemia", said to be caused by HTLV-I, it "is a real soup". However,
given the existence of endogenous retroviruses, when one uses
lymphocytes from normal individuals and umbilical cord lymphocytes, the
result is still "a real soup". Maybe a different soup, but nonetheless
still "a real soup".
A14. We agree that patients with AIDS and those at risk are
infected with a "stack of things". Furthermore, the cultures with
tissues from these patients in addition to these agents may also be
infected in vitro with other agents, such as mycoplasma.
A15. It may be true that sometimes it is easier to detect a
particle with the morphological characteristics of retrovirus in the
culture than in the plasma. However, since the viral "concentrate" is
obtained from the culture supernatant and since by definition a
"concentrate" would have more particles per unit volume than the culture
supernatant, it follows that it should be much easier to see a particle
in the concentrate than in the culture. Since Montagnier and his
colleagues "saw nothing major" in the "concentrate", that is, in the
1.16g/ml band, then why in their 1983 paper did they state the
"concentrate" not only contained viral particles but "purified" virus?
In the electron microscope picture which Montagnier and his associates
including Charles Dauget published there are buds on the cell surface,
some of which are more pronounced than others. But what is the evidence
that they are virus or they are in the process of becoming a virus?
A16. We agree it could be anything.
A17. We agree that familiarity may sometimes enable one to
distinguish between retroviral-like particles and other viral-like
particles using morphological features. However, there are particles
which are NOT viruses (including retroviruses) that exhibit identical
morphological features as retroviruses. Therefore from morphological
considerations both the buds and cell-free particles cannot be
considered to be retroviruses. Furthermore, cultures of tissues derived
from AIDS patients contain a plethora viral-like particles with
diameters ranging from 65-250nM, shapes which are spherical, angular and
tear drop, surfaces with and without spikes, and which contain cone
shaped, bar shaped, centrosymmetric and tubular cores, as well as double
cores and a mixture of cores. Like the several particles of varying
taxonomy deemed the HIV particle, none of these particles have been
purified and characterised and, like HIV, their origin and role must
remain conjecture.(9,61-64)
A18. 1. If they did not purify the particles why did they
claim to have done so and continue with the same claim up to this
interview?
2. It is true that they reported the peak of RT activity at the
density of 1.16g/ml, that is, at the density in which they claimed to
have "purified, labeled virus". However, how is it possible to claim
that the RT activity "was soundly that of a retrovirus", when they
"didn't take the peak...or it didn't work", that is when at that peak
they did not even find retrovirus-like particles, not to mention
retroviruses? To pass a retrovirus from one culture to another, one must
first have proof for the existence of a retrovirus in the first culture.
"Passing" non-specific phenomena is no proof for passing a retrovirus.
Furthermore, since all the phenomena which Montagnier and his colleagues
considered as proof for the existence of a retrovirus, including RT
activity and virus-like particles, could arise de novo in the
cultures, especially under the culture conditions they used, they cannot
claim proof for passing anything. How did Montagnier and his colleagues
know that if they had suitable controls, the same phenomena would not
have occurred in the blood donor's culture as well as the umbilical
lymphocytes even if they were not "infected" with "HIV"?
A19. 1. If the stage of purification (isolation) is not
necessary, then why did Montagnier and his colleagues claim to have
proven the existence of "HIV" because they "isolated" it, "purified" it?
2. Since any piece of DNA can be cloned and amplified, cloning and
amplifying a piece of DNA provides no information whatsoever in regard
to its origin, that is, if it is retroviral or not. Neither is it
possible by sequencing a piece of DNA to say that it is "truly a
retrovirus" unless prior proof exists that these sequences are present
in a retroviral particle and nowhere else. There is nothing specific
about the "structure of retroviruses". If indeed there is a unique
"sequence of DNA" indicating "it is truly a retrovirus" and "all the
retroviruses have a familiar genomic structure with such and such a
gene", then no such proof exists for the "HIV genome".(32). Suffice to
mention that to date no two identical sequences for the "HIV genome"
have been published. One and the same patient may have different "HIV
DNA" sequences. According to researchers from the Pasteur Institute, "an
asymptomatic patient can harbour at least 106 genetically
distinct variants of HIV, and for an AIDS patient the figure is more
than 108.(65-66) The genetic differences may reach 40%.(67) (Compare
this to the 1-2% differences between hominid DNAs, some of which code
for identical proteins such as haemoglobin a and b chains of chimpanzees
and humans). The length of the "HIV DNA" has been reported to be between
9-15Kb. In 1985 the Pasteur researchers reported that "The deduced
genetic structure is unique; it shows, in addition to the retroviral
gag, pol, and env genes, two novel open reading
frames we call Q and F".(68) In 1990 the "HIV" genome was said to
consist of ten genes,(69) in 1996 Montagnier reported that "HIV"
possesses eight genes (70) and, according to Barre-Sinoussi, (71) "HIV"
has nine genes.
A20. 1. For isolation of retroviruses the stage of
purification IS obligatory. One CANNOT ISOLATE retroviruses WITHOUT
PURIFYING. By definition, isolation means "to place apart or alone"
(Concise Oxford Dictionary) and purify means "to clear of foreign
elements" (Concise Oxford Dictionary). Thus, unless the
contaminants are removed from around the "HIV" particles (that is, to
purify the "HIV"), the "HIV" particles are NOT ISOLATED.
2. We agree that to transmit a retrovirus one does not need pure
material. However, to transmit something, one first must know what one
is transmitting, that is, one must have proof for its existence. For
retroviruses such evidence can only be obtained by isolating (purifying)
the particles, determining their physical and chemical properties and
proving they are infectious.
A21. Yes, it is impossible to determine the identity of the
proteins including that of RT without isolation. 1. Montagnier and his
colleagues, even after a Roman effort could not find even
retrovirus-like particles at this density thus, from his experience
(experimental evidence), there are zero chances and NOT 999 out of 1000
that RT activity at the density of 1.15, 1.16 represents a retrovirus in
their case.
2. We agree that it could be a retrovirus of different origin. The
existence of endogenous retroviruses, together with the presence in AIDS
patients and those at risk of antibodies which react with their
antigens, means that even if Montagnier et al had proven the
existence of a retrovirus, it would have been impossible to say that the
retrovirus originated in the gay man and not in the donors or umbilical
cord lymphocytes.
3. The "molecular biology", the "cloning and sequencing" of the "HIV"
genome has been discussed in detail elsewhere.(32-49) Suffice to mention
here that:
(a) proof for the existence of "HIV" and indeed for its causative
role in AIDS was claimed before any "molecular biology", "cloning and
sequencing";
(b) since any piece of nucleic acid can be cloned and sequenced,
cloning and sequencing of a piece of nucleic acid cannot be used to
prove the existence of a retrovirus or of its genome. To the contrary,
proof for the existence of viral nucleic acids (viral RNA and cDNA) can
be accepted if and only if it is shown that the RNA is a unique
molecular entity belonging to particles with morphological, physical and
replicative characteristics of retroviral particles. This can only be
done by separating the particles from everything else, by purifying
them. Instead, Montagnier and Gallo used "a real soup" of cultures and
co-cultures (Montagnier's group even purposely infected the cultures
with Epstein-Barr virus). The supernatant from these cultures was banded
in sucrose density gradients. From all the RNA (and DNA) which banded at
1.16g/ml they arbitrarily chose some RNA using totally non-retroviral
specific criteria and called it "HIV RNA", without any proof that the
band contained even retroviral like particles;(32)
(c) the first, absolutely necessary step in proving that the "HIV
RNA", retroviral or not, originated from the lymphocytes of "HIV"
infected individuals, is to perform hybridisation experiments using
fresh, uncultured lymphocytes and the "HIV DNA" (obtained by reverse
transcription of the "HIV RNA"), as a probe. It is hard to understand
why Montagnier and his colleagues did not report such experiments.
Gallo's group did and the results were negative. In 1994 Gallo was
quoted in this magazine as saying: "We have never found HIV DNA in the
tumour cells of KS...In fact we have never found HIV DNA in
T-cells".(72) At present there is no study proving the existence of even
one single copy of the "full-length HIV genome" in the fresh T-cells
even of a single AIDS patient or a patient at risk of AIDS; (d)
Currently the number of "HIV" particles in the plasma is quantified by
measuring "HIV RNA", the viral load which is reported to be "15 x
103 to 554 x 103 virions per ml".(73) Many studies
claim proof that the "viral load", the "HIV RNA", can be decreased to
undetectable levels by the use of both RT and protease inhibitors.
However, since: (i) it is accepted that the "HIV RNA" is a transcript of
the "HIV DNA"; (ii) by their nature neither the RT nor the protease
inhibitors have any effect on DNA transcription, they only inhibit
infection of new cells, that is, the decrease in "HIV RNA" is a
consequence of the decrease in "HIV DNA"; one would expect that the
effect of these drugs would be determined by measuring the level of "HIV
DNA". Yet hardly any such studies have been published. The very few
which exist show that neither RT nor protease inhibitors have any effect
on "HIV DNA",(74-76) which means that no relationship exists between
"HIV RNA" and "HIV DNA".
4. In 1984 Montagnier and his colleagues reported that "preincubation
of T4+ lymphocytes with three different monoclonal antibodies directed
at the T4 glycoprotein blocked cell infection by LAV", that is, blocked
the detection of RT activity in T4 cells "infected" with "HIV". They
concluded their "findings strongly suggest that the T4 glycoprotein is
at least associated with all or part of the receptor for LAV".(38).
However, blocking a non-specific "HIV" phenomena, namely RT activity,
cannot be considered proof of blocking "HIV" infection or association of
"HIV" with T4 cells.
A22. We agree that "analysis of the proteins of the virus
demands mass production and purification. It is necessary to do that".
In this respect they have not just partially failed, but TOTALLY FAILED.
If the "analysis of the proteins of the virus demands mass production
and purification", so does the analysis of "nucleic acids, cloning etc".
If one fails to purify the virus then it fails:
(a) to characterise the viral antigens and to obtain a gold standard
for the antigen-antibody reaction, that is, one cannot use antibody
tests to define infection with the retrovirus;
(b) to obtain and characterise the retroviral nucleic acids, RNA
(cDNA) and thus probes and primers for hybridisation and PCR studies,
that is, one cannot use molecular tests to define retroviral infection.
That this is the case is accepted by Donald Francis, a researcher who
with Gallo, played a significant role in developing the theory that AIDS
is caused by a retrovirus. In 1983, Francis, then the chief collaborator
of the AIDS Laboratory Activities, US Centers for Disease Control and
former chief of the WHO smallpox program, speculated on a viral cause
for AIDS: "One must rely on more elaborate detection methods through
which, by some specific tool, one can "see" a virus. Some specific
substances, such as antibody or nucleic acids, will identify viruses
even if the cells remain alive. The problem here is that such methods
can be developed only if we know what we are looking for. That is, if we
are looking for a known virus we can vaccinate a guinea pig, for
example, with pure virus... Obviously, though, if we don't know
what virus we are searching for and we are thus unable to raise
antibodies in guinea pigs, it is difficult to use these methods...we
would be looking for something that might or might not be there using
techniques that might or might not work"(77) (italics ours).
A23. It is impossible to characterise two viral unknowns,
namely its proteins and the antibodies directed against them, by the
formation of an antibody/antigen complex let alone characterise the
"virus". By what means did Montagnier know that somebody had antibodies
against the proteins of the virus and that the proteins with which the
antibodies react were viral? It is a scientific impossibility to know
that somebody has antibodies to a virus and at the same time, the
1.16g/ml band contains proteins of the same virus before one has proven
its existence.
A24. 1. It is true that Montagnier had controls but the
controls were not suitable. Montagnier and his colleagues reacted the
proteins which banded at 1.16g/ml with the sera from two gay patients
with lymphadenopathy. The patients with AIDS and those at risk were
already known to have a plethora of antibodies, all with potential for
cross-reactivity. Therefore, one would have expected that Montagnier
et al to have used as controls sick individuals who did not have
AIDS or pre-AIDS and who were not at risk for AIDS but who also had a
plethora of antibodies, all with potential for cross-reactivity. Instead
their controls consisted of two blood donors whose state of good health
is characterised by lower levels of antibodies.
2. Montagnier et al did not obtain proof for "a specific
reaction". The sera from the patients and the donors were reacted with
both the "purified virus", that is the 1.16g/ml band, and extracts from
the "infected" cells. In their published strips, with "purified virus",
it is not possible to distinguish any reacting proteins with any of the
sera. In the text they state: "When purified, labelled virus [the
1.16g/ml band] from patient 1 was analysed...three major proteins could
be seen; the p25 protein and proteins with molecular weight of 80.000
and 45.000". No such reactions were reported with the donors' sera. In
the published strips with extracts from the "infected cells", it is
obvious that many proteins reacted with both the patients' and the
healthy blood donors' sera. One year later Montagnier and his colleagues
confirmed that "sera from some AIDS patients bound a lot of cellular
proteins...This banding was apparent in the RIPA and only sera which
specifically precipitated the p25 were regarded as positive". In other
words, for some unknown reason, they concluded that from all the
reacting proteins only p24 (their p25) was retroviral and from all the
antibodies only the one which reacted with p24 was directed against the
retrovirus. Even if one considers the reaction between the p24 which
bands at 1.16g/ml and the antibody present in the sera specific, that
is, not due to cross-reactivity, from such a reaction it is impossible
to draw the conclusion that p24 is retroviral protein and the antibody
is elicited as a result of infection with this retrovirus. Indeed given
the fact that Montagnier et al could not even detect
retrovirus-like particles at 1.16g/ml, their conclusions regarding p24
and the antibody reacting with it completely defies scientific
reasoning.
A25. 1. No antibodies, not even monoclonal antibodies are
"very specific" or even specific.(78-84) Indeed, there are instances
where "cross-reactive antigen binds with higher affinity than the
homologous antigen itself...The most obvious fact about cross-reactions
of monoclonal antibodies is that they are characteristic of all
molecules and cannot be removed by absorption without removing all
reactivity...Even antigens that differ for most of their structure can
share one determinant, and a monoclonal antibody recognising this site
would then give a 100% cross-reaction. An example is the reaction of
autoantibodies in lupus with both DNA and cardiolipin".(80)
However, "It should be emphasised that sharing a "determinant" does
not mean that the antigens contain identical chemical structures, but
rather that they bear a chemical resemblance that may not be well
understood, for example, a distribution of surface charges".(80) It is
of importance to note that "HIV" experts concede "cross-reactivity" as
the reason for "indeterminate" antibody reactivity seen in the "HIV"
Western blot, as well as, for example, reactivity between monoclonal
antibodies to the "HIV" p18 protein and dendritic cells in the lymphatic
tissues of a variety of patients with a number of non-AIDS related
diseases (85) and normal tissues taken from "non-HIV" infected
individuals.(86) For one to be convinced that all "antibodies [including
monoclonal] are polyspecific, that is, they are able to react with
various dissimilar antigens such as: proteins, nucleic acids and
haptens", "they are able to react with more than to self or non-self
antigens, often without any apparent antigenic similarities", all one
has to do is to read the scientific publications of the researchers from
the Pasteur Institute such as Stratis Avrameas.(83-87)
2. It cannot be concluded that a protein which bands at 1.16g/ml is
viral merely because it reacts with an antibody present in the patient's
sera even if somehow one knows that the antibodies present in the sera
are monoclonal. Let us assume an ideal situation where: (a) all the
antibodies present in the patients' sera are monoclonal and "very
specific"; (b) the 1.16g/ml band contains in addition to the many
unembodied and microvesicles, embodied proteins of cellular origin and
maybe of bacterial, fungal and viral origin (constituents of the many
infectious agents, other than retroviruses, present in the culture and
the patients) and, as shown in a 1997 France/German study, a number of
retrovirus-like particles. Even in this ideal situation, it is NOT
POSSIBLE TO CLAIM that just because a protein such as p24, p41, or
others is found in this band and reacts with the sera, the protein is a
constituent of the retrovirus-like particles.
3. The reality is that: (a) all AIDS patients and those at risk have
a plethora of antibodies including auto-antibodies. The auto-antibodies
include anti-lymphocyte, and as Montagnier and his colleagues have
shown88 anti-actin and anti-myosin antibodies, that is antibodies to the
two ubiquitous cellular proteins actin and myosin. (b) all the
antibodies present in the sera have the potential of cross-reactivity.
(c) the proteins from the supernatant of non-infected lymphocytes which
in sucrose density gradients band at 1.16g/ml, the mock virus, include
proteins having the same molecular weights as the "HIV" proteins;89 (d)
animals inoculated with the mock virus develop antibodies which react
with the "SIV" proteins, a "retrovirus" whose proteins share the same
molecular weights as the "HIV" proteins and is said to be the closest
relative of "HIV";90 (e) AIDS patients and those at risk are repeatedly
subjected to allogenic stimuli including allogenic lymphocytes; (f) up
till 1997 no evidence existed showing that the 1.16g/ml band contained
even retrovirus-like particles. Given this reality, to claim that just
because a protein bands at 1.16g/ml and reacts with antibodies present
in the patients' sera is at best no different than the following: (i) A
researcher has two bowls, one of them contains a mixture of raw eggs,
some known and maybe some unknown, and maybe some milk originating from
several animals. The other contains several acids. Again some known and
maybe some unknown. Once the contents of the two bowls are mixed he gets
a precipitate. He claims that the precipitation proves the existence in
the bowl of milk from a previously unknown animal and an unknown acid
and that the reaction is between the unknown acid and a protein of the
previously unknown milk. (ii) This claim is scientifically impossible
since any protein in the eggs could have reacted with any acid to
produce the observed precipitate.
Thus, given the reality as outlined in (a) to (f) above, it is
completely unscientific to claim that the reaction between proteins
which band at 1.16g/ml and react with antibodies present in the
patients' sera is proof of the existence of "HIV". To claim that the
reaction between proteins which band at 1.16g/ml (in the absence of
evidence that the band contains even retrovirus-like particles) with
antibodies present in the sera indicates not only the band contains
retroviral proteins, but proteins of a new retrovirus, is no different
than the following: A fisherman who has sea creatures but no fish in a
net. He throws some animals into the net. The fisherman observes that
the animals eat some proteins present in the net and claims that the
proteins were not just fish proteins but the proteins of a completely
new fish, a fish which nobody has seen before, a golden fish.
A26. 1. It is not possible for both Montagnier and Gallo to be
"reasonably right". Both Gallo and Montagnier reacted the 1.16g/ml band
with patient sera. Irrespective of the method used to detect the
reaction (RIPA or WB), or the number of reactions performed, they should
have found the same reacting proteins.
2. In their 1983 study, Montagnier and his colleagues found three
proteins, p25, p45 and p80. Regarding p45 they wrote: "The 45K protein
may be due to contamination of the virus by cellular actin which was
present in immunoprecipitates of all the cell extracts". In a study
published in 1984 they had "a prominent p25, a p18, a low molecular
weight protein at the bottom of the gel (p12), and three proteins of
high molecular weight (43.000, 53.000, 68.000). The band at 43.000 may
include a component of cellular origin, since it was also found in a
similar preparation made from the control uninfected cells".
3. Since both patients' and healthy blood donors' sera repeatedly
reacted with the p45/p43 protein from both infected and in-infected
cells one would have expected Gallo to also detect this protein. However
neither Gallo nor anybody else since then reported such a band
irrespective of the method used to detect the antigen/antibody reaction.
The discrepancy can be resolved if one takes into consideration the fact
that the migration of proteins in an electrophoretic strip, in addition
to the molecular weight, may be also influenced by other factors, for
example the charge carried by the protein. Thus one and the same protein
may appear to have slightly different molecular weight when detected by
either RIPA or the WB. For example, both p25 detected by Montagnier and
the p24 detected by Gallo at present are considered to be both one and
the same "HIV" protein p24.
4. The molecular weight of actin is neither 45,000 nor 43,000 but
41,000. At present there is ample evidence that the 1.16g/ml band the
"Pure HIV" contains cellular actin (91-94) and as has been already
mentioned Montagnier himself showed that the sera of AIDS patients and
those at risk contain antibodies which react with actin. In other words
when the 1.16g/ml band is reacted with patients' sera, irrespective of
the presence of "HIV", a p41 (p45/43) band must be present, and
represent cellular actin. (If Montagnier now believes that p41 is an
"HIV" protein, why does he persist in excluding this band from his
criteria for a positive Western blot?(95))
A27. The p24 protein is not sufficient for diagnosing "HIV"
infection because it is not specific. Indeed, no other "HIV" protein not
even p41 (p45/43) has been reported to react more often with sera from
healthy (at no risk of AIDS) individuals. Neither has a monoclonal
antibody to any of the other "HIV" proteins been found to react more
often with proteins present in non "infected" cultures or sera from
individuals at no risk of AIDS. According to Montagnier because: (a)
"these are cellular proteins that one meets everywhere - there is a
non-specific background noise"; (b) one such protein, having a molecular
weight of 45/43, is actin; (c) this protein reacted with sera from
individuals at no risk of AIDS; the p45/43 represents a cellular and not
a viral protein. However, since: (i) myosin is as ubiqitous as actin.
(ii) myosin has a light chain with a molecular weight of 24,000. (iii)
the cytoskeletal proteins (of which actin and myosin are the most
abundant) have been reported in "pure HIV".(91-94) Indeed, myosin and
actin are said to play a crucial in budding and release of the "HIV"
particles.(91) (iv) Montagnier has shown that patients with AIDS and at
risk of AIDS have anti-myosin antibodies. Why should not one consider
the p24 band as representing myosin?
A28. We agree that no protein is sufficient to diagnose "HIV"
infection. The problem then, as it is today, was not "to know whether it
was an HTLV or not", but whether it was retroviral or not. Not
everything which is not HTLV is retroviral.
A29. 1. To date there is no proof that any of the proteins
which band at 1.16g/ml are "HIV" proteins. The only reason that 20% of
the proteins which band at 1.16g/ml are said to be "HIV" is that this
fraction of proteins is found to react with different AIDS patient's
sera at some time or another.
2. We agree that with the technique used by Montagnier's group, one
cannot prove which proteins (or nucleic acids) are cellular and which
are viral.
3. We agree. The only way one can prove the existence of the viral
protein (nucleic acids) is "to purify the virus to the maximum", that
is, to obtain density gradients which contain only particles with the
morphological characteristics of retrovirus and nothing else. This has
never been done to prove the existence of the "HIV" proteins and nucleic
acids.
4. If one always "stumbles on the same proteins" in successive
gradients, this is no proof that these proteins are viral and the ones
which disappear are cellular.
A30. 1. No matter how many times the banding is repeated, if
one starts with no retrovirus-like particles one will end with no such
particles. Some times, by successive bandings, one may be able to
eliminate non-retroviral components and obtain a band which contains
nothing else but particles with morphological characteristics of
retroviruses. However, to be able to do so, even after the first
banding, one must begin with a relatively high proportion of
retrovirus-like particles.
2. Once again, the origin of the proteins cannot be determined by
molecular analysis, that is, by sequencing the proteins.
3. We agree that if the proteins of a retrovirus are coded by its
genome, as is generally accepted, then it may be possible to
characterise the retroviral proteins by its genome. However, to do this
one must first prove that the RNA (cDNA) is a constituent of a
retroviral particle. This has not been done for the "HIV" genome. In
fact even today there is no proof that the "HIV" RNA is a constituent of
a particle, any particle viral or non viral.
4. To date there is no proof of a relationship between the sequences
in the "HIV" RNA (DNA) and the sequences in the proteins "observed with
immunoprecipitation or with gel electrophorosis". In fact there is no
relationship even between the size of the proteins coded by the "HIV"
genes and the size of the proteins "observed with immunoprecipitation or
with gel electrophorosis". For example, in 1987 Gallo and his associates
performed a "computer-assisted analysis" of the "amino acid sequences of
the envelope protein complexes derived from the nucleic acid sequences
of seven AIDS virus isolates", and concluded that "gp41 should be about
52 to 54 daltons by calculation".(96)
5. One of the many puzzling aspects of "HIV" is the following: (a)
"HIV" experts agree that no two "HIVs" have the same genomic sequences
and the difference may be as high as 40%;(67) (b) They also admit that
the vast majority (99.9%) of the "HIV" genomes are defective, that is,
either part of a gene(s) or whole gene(s) are missing; How then is it
possible: (i) to measure the viral burden ("HIV DNA") and the viral load
("HIV RNA") by using one and the same hybridisation probes and PCR
primers? (ii) to perform antibody tests by using kits containing the
same antigens for all the different "HIVs"?
6. Indeed, the history as to how "HIV" researchers have tried to
prove the existence of p120 and how they ultimately agreed on its
existence is very interesting and informative.32 However, given the fact
that the p120 protein is said to be present only in the knobs, no
cell-free "HIV" particles possessing knobs have been reported so far. It
follows neither the particles in the culture supernatant nor the "pure"
virus will have gp120. In other words, it is impossible for either the
RIPA or the WB strips to have a "HIV" protein of molecular weight
120,000.
A31. No such proof can be found in the published
literature.
A32. 1. Prior to March 1997 no group of "HIV" researchers had
published even a single electron micrograph of material banding at the
density of 1.16gm/ml in a sucrose density gradient. The first EMs of
material banded in sucrose density gradients appeared in 1997 in two
publications, one Franco/German and the other from the US National
Cancer Institute (NCI).(89) The Franco/German EMs are from the 1.16
gm/ml sucrose density gradient whereas it is not possible to tell from
which density the NCI data originate. The data from both studies reveal
that the vast majority of the material is "non-viral", "mock" virus,
cellular "microvesicles", that is, the banded material is virtually all
cellular. These particles, like the retroviral particles, contain
nucleic acids in addition to proteins but they are not as condensed.
2. The EM micrographs in both studies also contain a small minority
of particles which have morphologies more closely resembling retroviral
particles than the "mock" particles. Both groups claim the fewer
particles are "HIV".
3. In the NCI study no reasons are given for the claim that these
particles are "HIV". The authors of the Franco/German study claim that
the particles are "HIV" because they have: (a) "diameters of about
110nm;" (b) a "dense cone-shaped core"; (c) "lateral bodies"; and
because no such particles were seen in the banded material from the
non-"infected" control cells. However, according to well known
retroviral researchers such as Bader and Frank, one type of "oncoviral
particle" can change to another, and immature cores to "mature", merely
by changing the extracellular conditions.(11-97) However the culture
conditions in the "infected" and non-infected cells were not the same. A
diameter of 100-120nm and surface knobs are two morphological
characteristics shared by all retroviruses. None of the particles appear
to have knobs and none has a diameter of less than 120nm. Averaging the
major and minor diameters of the particles indicated and said to
represent "HIV" and, assuming all particles are spherical, shows that in
the Franco/German study the particles are 1.14 times larger than bona
fide retroviral particles and the NCI particles are 1.96 times larger.
These data translate into volumes 50% and 750% greater respectively.
Since density is the ratio of mass to volume these particles must
therefore have correspondingly higher masses. Given the maximum diameter
of retroviral particles and the fact that such particles contain a fixed
mass of RNA and protein, it appears untenable that the particles which
both groups regard as "HIV" are the same particle or are retroviral
particles. The only other explanation for these data is that the
electron micrographs are not from the 1.16gm/ml band or the banding has
not been to equilibrium in which case one must redefine the buoyant
density of retroviruses.
The "HIV" particles are said to have a cone shaped viral core, with
dense lateral bodies at either side of the core. No such feature can be
seen in the EM published in these two studies. Thus, by definition, the
particles cannot even be said to be retrovirus-like.
Taking into consideration that in both studies the control
"non-infected" cultures were of H9 cells and the fact that Gallo as far
back as 1983 claimed that these cells are infected with HTLV-I, the
non-reporting of virus-like particles in the banded material from these
cultures is an enigma.
A33. Pictures of the 1.16g/ml are of profoundly significant
interest. How else can it be known that there are retrovirus-like
particles there, especially since even Montagnier admits that other
things may band there. For any scientist who claims proof for isolation,
purification of a retrovirus using sucrose density gradient banding, it
is vital and absolutely necessary to obtain electron micrographs of the
1.16g/ml band showing nothing else but retrovirus-like particles.
A34. If this is the case why is such data not available in the
scientific literature?
A35. In one of their 1984 papers (22) Montagnier and his
colleagues wrote, "Several characteristics indicate that LAV or LAV
related viruses belong to the retroviruses family. Budding particles at
the plasma membrane have been observed in electron microscopy. The
density of the virus in sucrose gradient is 1.16 and a Mg2+ dependent
reverse transcriptase activity has been found to be associated with RNA
containing virions". However, in this interview Montagnier admits: (a)
"We published images of budding which are characteristic of
retroviruses. Having said that, on the morphology alone one could not
say it was truly a retrovirus... With the first budding pictures it
could be a type C virus. One cannot distinguish... No... well, after
all, yes... it could be another budding virus". (b) at the sucrose
density of 1.16 gm/ml not only did Montagnier and his colleagues not see
a retrovirus particle, they repeatedly said they did not see
retroviral-like particles; (c) although at the sucrose density of 1.16
gm/ml they detected reverse transcription of the template primer
An.dT12-18 in the presence of Mg2+, they had no particles and thus no
evidence for "reverse transcriptase activity found to be associated with
RNA containing virions".
Furthermore, in this study (22), they showed that DNA polymerases
beta and gamma and of non-infected cells reverse transcribe An.dT
(12-18) in the presence of Mg2+. Thus, Montagnier's own conditions and
data do not prove his claim that what he has "seen" and "encountered" is
a retrovirus. If "HIV" "exists", and it is "clear" to Montagnier that he
has "seen it" and "encountered it", where is his proof? *
Eleni Papadopulos-Eleopulos (1) Valendar F.Turner (2) John M.
Papadimitriou (3) Barry Page (1) & David Causer (1)
(1)
Department of Medical Physics, (2) Department of Emergency Medicine,
Royal Perth Hospital, Perth, Western Australia; (3) Department of
Pathology, University of Western Australia.
References:
1. Rous P. A Sarcoma of the Fowl transmissible by an
agent separable from the Tumor Cells. J. Exp. Med.
1911;13:397-411.
2. Boycott AE. The transition form life to death; the
nature of filterable viruses. Proc. Royal Soc. Med.
1928;22:55-69.
3. Darlington C. The plasmagene theory of the origin of
cancer. Br. J. Cancer 1948;2:118-126.
4. Papadopulos-Eleopulos E. A Mitotic Theory. J. Theor.
Biol. 1982;96:741-758.
5. Papadopulos-Eleopulos E. Reappraisal of AIDS: Is the
oxidation caused by the risk factors the primary cause? Med. Hypotheses
1988;25:151-162.
6. Papadopulos-Eleopulos E, Turner VF, Papadimitriou
JM. Oxidative Stress, HIV and AIDS. Res. Immunol.
1992;143:145-148.
7. Papadopulos-Eleopulos E, Turner VF, Papdimitriou JM.
Is a Positive Western Blot Proof of HIV Infection? Bio/Technology
1993;11:696-707.
8. Beard JW, Sharp DG. Virus of avian
erythromyeloblastic leukosis. Biochemia and Biophysica Acta
1954;14:12-17.
9. Gelderblom HR, Özel M, Hausmann EHS, Winkel T, et
al. Fine Structure of Human Immunodeficiency Virus (HIV),
Immunolocalization of Structural Proteins and Virus-Cell Relation.
Micron Microscopica 1988;19:41-60.
10. Beard JW. Physical methods for the analysis of
cells. Ann. N. Y. Acad. Sci. 1957;69:530-544.
11. Bader JP. Reproduction of RNA Tumor Viruses. In:
Fraenkel-Conrat H, Wagne RR, ed. Comprehensive Virology. New York:
Plenum Press, 1975: 253-331. vol 4).
12. Toplin I. Tumor Virus Purification using Zonal
Rotors. Spectra 1973: 225-235.
13. Temin HM, Baltimore D. RNA-Directed DNA Synthesis
and RNA Tumor Viruses. Adv. Virol. Res. 1972;17:129-186.
14. Sinoussi F, Mendiola L, Chermann JC. Purification
and partial differentiation of the particles of murine sarcoma virus (M.
MSV) according to their sedimentation rates in sucrose density
gradients. Spectra 1973;4:237-243.
15. Toyoshima K, Vogt PK. Enhancement and Inhibition of
Avian Sarcoma Viruses by Polycations and Polyanions. Virol.
1969;38:414-426.
16. Aaronson SA, Todaro GJ, Scholnick EM. Induction of
murine C-type viruses from clonal lines of virus-free BALB/3T3 cells.
Science 1971;174:157-159.
17. Hirsch MS, Phillips SM, Solnik C. Activation of
Leukemia Viruses by Graft-Versus-Host and Mixed Lymphocyte Reactions In
Vitro. Proc. Natl. Acad. Sci. U S A 1972;69:1069-1072.
18. Barré-Sinoussi F, Chermann JC, Rey F. Isolation of
a T-Lymphotrophic Retrovirus from a patient at Risk for Acquired Immune
Deficiency Syndrome (AIDS). Science 1983;220:868-871.
19. Lee MH, Sano K, Morales FE, Imagawa DT. Sensitive
reverse transcriptase assay to detect and quantitate human
immunodeficiency virus. J. Clin. Microbiol. 1987;25:1717-21.
20. Brun-Vezinet F, Rouzioux C, Barre-Sinoussi F,
Klatzmann D, et al. Detection of IgG antibodies to
lymphadenopathy-associated virus in patients with AIDS or
lymphadenopathy syndrome. Lancet 1984;i:1253-6.
21. Vilmer E, Rouzioux C, Vezinet Brun F, Fischer A, et
al. Isolation of new lymphotropic retrovirus from two siblings with
Haemophilia B, one with AIDS. Lancet 1984;i:753-757.
22. Rey MA, Spire B, Dormont D, Barre-Sinoussi F, et
al. Characterization of the RNA dependent DNA polymerase of a new human
T-lymphotropic retrovirus (lymphadenopathy associated virus). Biochem.
Biophys. Res. Commun. 1984;121:126-33.
23. Gallo RC, Wong-Staal F, Reitz M, Gallagher RE, et
al. Some evidence for infectious type-C virus in humans. In: Balimore D,
Huang AS, Fox CF, eds. Animal Virology. New York: Academic Press Inc.,
1976: 385-405.
24. Varmus H. Reverse Transcription. Sci. Am.
1987;257:48-54.
25. Varmus H. Retroviruses. Science
1988;240:1427-1435.
26. Gallo RC, Sarin PS, Wu AM. On the nature of the
Nucleic Acids and RNA Dependent DNA Polymerase from RNA Tumor Viruses
and Human Cells. In: Silvestri LG, ed. Possible Episomes in Eukaryotes.
Amsterdam: North-Holland Publishing Company, 1973: 13-34.
27. Tomley FM, Armstrong SJ, Mahy BWJ, Owen LN. Reverse
transcriptase activity and particles of retroviral density in cultured
canine lymphosarcoma supernatants. Br. J. Cancer
1983;47:277-284.
28. Temin HM. On the origin of RNA tumor viruses.
Harvey Lect. 1974;69:173-197.
29. Gallagher RE, Gallo RC. Type C RNA Tumor Virus
Isolated from Cultured Human Acute Myelogenous Leukemia Cells. Science
1975;187:350-353.
30. Papadopulos-Eleopulos E, Turner VF, Papadimitriou
JM, Causer D. A critical analysis of the evidence for the isolation of
HIV. At Website http://www.virusmyth.com/aids/data/epappraisal.htm
1997.
31. Papadopulos-Eleopulos E, Turner VF, Papadimitriou
JM, Causer D. HIV antibodies: Further questions and a plea for
clarification. Curr. Med. Res. Opin. 1997;13:627-634.
32. Papadopulos-Eleopulos E, Turner VF, Papadimitriou
JM, Causer D. The Isolation of HIV: Has it really been achieved?
Continuum 1996;4:1s-24s.
33. Lower R, Lower J, Kurth R. The viruses in all of
us: Characteristics and biological significance of human endogenous
retrovirus sequences. Proc. Natl. Acad. Sci. U S A
1996;93:5177-5184.
34. Marx JL. Human T-cell virus linked to AIDS. Science
1983;220:806-809.
35. Gougeon ML, Laurent-Crawford AG, Hovanessian AG,
Montagnier L. Direct and indirect mechanisms mediating apoptosis during
HIV infection: contribution to in vivo CD4 T cell depletion. Immunol.
1993;5:187-194.
36. Cohen J. Exploiting the HIV-chemokine nexus.
Science 1997;276(5315):1261-1264.
37. Layne SP, Merges MJ, Dembo M, Spouge JL, et al.
Factors underlying spontaneous inactivation and susceptibility to
neutralization of human immunodeficiency virus. Virol.
1992;189:695-714.
38. Klatzmann D, Barré-Sinoussi F, Nugeyre MT.
Selective Tropism of Lymphadenopathy Associated Virus (LAV) for
Helper-Inducer T Lymphocytes. Science 1984;225:59-63.
39. Klatzmann D, Montagnier L. Approaches to AIDS
therapy. Nature 1986;319:10-11.
40. Acres RB, Conlon PJ, Mochizuki DY, Gallis B. Rapid
Phosphorylation and Modulation of the T4 Antigen on Cloned Helper T
Cells Induced by Phorbol Myristate Acetate or Antigen. J. Biol. Chem.
1986;261:16210-16214.
41. Zagury D, Bernard J, Leonard R, Cheynier R, et al.
Long-Term Cultures of HTLV-III-Infected T Cells: A Model of
Cytopathology of T-Cell Depletion in AIDS. Science
1986;231:850-853.
42. Scharff O, Foder B, Thastrup O, Hofmann B, et al.
Effect of thapsigargin on cytoplasmic Ca2+ and proliferation of human
lymphocytes in relation to AIDS. Biochim. Biophysica. Acta.
1988;972:257-264.
43. Birch RE, Rosenthal AK, Polmar SH. Pharmacological
modification of immunoregulatory T lymphocytes. II. Modulation of T
lymphocyte cell surface characteristics. Clin. Exp. Immunol.
1982;48:231-238.
44. Zagury D, Bernard J, Leonard R, Cheynier R, et al.
Long-Term Cultures of HTLV-III-Infected T Cells: A Model of
Cytopathology of T-Cell Depletion in AIDS. Science 1986;231(21st
February):850-853.
45. Laurent-Crawford AG, Krust B, Muller S, Rivière Y,
et al. The Cytopathic Effect of HIV is Associated with Apoptosis. Virol.
1991;185:829-839.
46. Dourmashkin RR, O'Toole CM, Bucher D, Oxford JS.
The presence of budding virus-like particles in human lymphoid cells
used for HIV cultivation. VIIth International Conference on AIDS.
Florence: 1991:122.
47. O'Hara CJ, Groopmen JE, Federman M. The
Ultrastructural and Immunohistochemical Demonstration of Viral Particles
in Lymph Nodes from Human Immunodeficiency Virus-Related Lymphadenopathy
Syndromes. Human Pathology 1988;19:545-549.
48. Gallo RC, Sarin PS, Kramarsky B, Salahuddin Z, et
al. First isolation of HTLV-III. Nature 1986;321:119.
49. Duesberg PH. Peter Duesberg responds. Continuum
1996;4:8-9.
50. Nermut MV, Steven AC, ed. Retroviridae. Animal
Virus and Structure. Oxford: Elsevier, 1987
51. Gallo RC, Fauci AS. The human retroviruses. In:
Isselbacher KJ, Braunwald E, Wilson JD, Martin JB, Fauci AS, Kasper DL,
ed. Harrison's Principles of Internal Medicine. 13 ed. New York:
McGraw-Hill Inc., 1994: 808-814.
52. Todaro GJ, Benveniste RE, Sherr CJ. Interspecies
Transfer of RNA Tumour Virus Genes: Implications for the search for
"Human" Type C Viruses. In: Baltimore D, Huang AS, Fox CS, ed. Animal
Virology. New York: Academic Press Inc., 1976: 369-384.
53. Papadopulos-Eleopulos E, Turner VF, Papadimitriou
JM, Causer D, et al. A critical analysis of the HIV-T4-cell-AIDS
hypothesis. Genetica 1995;95:5-24.
54. Montagnier L. Lymphadenopathy-Associated Virus:
From Molecular Biology to Pathogenicity. Ann. Int. Med.
1985;103:689-693.
55. Duesberg PD. Inventing the AIDS Virus.Washington,
USA: Regnery Publishing, Inc., 1996.
56. Padian NS, Shiboski SC, Jewell NP. Female-to-male
transmission of human immunodeficiency virus. JAMA
1991;266:1664-7.
57. Brettler DB, Forsberg AD, Levine PH, Andrews CA, et
al. Human immunodeficiency virus isolation studies and antibody testing.
Household contacts and sexual partners of persons with hemophilia. Arch.
Int. Med. 1988;148:1299-301.
58. van der Ende ME, Rothbarth P, Stibbe J.
Heterosexual transmission of HIV by haemophiliacs. Brit. Med. J.
1988;297:1102-3.
59. Padian NS, Shiboski SC, Glass SO, Vittinghoff E.
Heterosexual transmission of human immunodeficiency virus (HIV) in
northern California: results from a ten-year study. Am. J. Epidemiol.
1997;146:350-7.
60. Burger H, Weiser B, Robinson WS, Lifson J, et al.
Transient antibody to lymphadenopathy-associated virus/human
T-lymphotropic virus type III and T-lymphocyte abnormalities in the wife
of a man who developed the acquired immunodeficiency syndrome. Ann. Int.
Med. 1985;103:545-7.
61. Hockley DJ, Wood RD, Jacobs JP. Electron Microscopy
of Human Immunodeficiency Virus. J. Gen. Virol.
1988;69:2455-2469.
62. Lecatsas G, Taylor MB. Pleomorphism in HTLV-III,
the AIDS virus. S. Afr. Med. J. 1986;69:793-794.
63. Palmer E, Sporborg C, Harrison A, Martin ML, et al.
Morphology and immunoelectron microscopy of AIDS virus. Arch. Virol.
1985;85:189-196.
64. Papadopulos-Eleopulos E, Turner VF, Papadimitriou
JM. Virus Challenge. Continuum 1996;4:24-27.
65. Vartian JP, Meyerhans A, Henry M, Wain-Hobson W.
High-resolution structure of an HIV-1 quasispecies: Identification of
novel coding sequences. AIDS 1992;6:1095-1098.
66. Wain-Hobson S. Virological mayhem. Nature
1995;373:102.
67. Kozal MJ, Shah N, Shen N, Yang R, et al. Extensive
polymorphisms observed in HIV-1 clade B protease gene using high-density
oligonucleotide arrays. Nat. Med. 1996;2:753-759.
68. Wain-Hobson S, Sonigo P, Danos O, Cole S, et al.
Nucleotide sequence of the AIDS virus, LAV. Cell
1985;40:9-17.
69. Lazo PA, Tsichlis PN. Biology and pathogenesis of
retroviruses. Semin. Oncol. 1990;17:269-294.
70. Cunningham AL, Dwyer DE, Mills J, Montagnier L.
Structure and function of HIV. Med. J. Aust.
1996;164:161-173.
71. Barré-Sinoussi F. HIV as the cause of AIDS. Lancet
1996;348:31-35.
72. Lauritsen JL. NIDA meeting calls for research into
the poppers-Kaposi's sarcoma connection. In: Duesberg PH, ed. AIDS:
Virus- or Drug Induced. London: Kluwer Academic Publishers, 1995:
325-330.
73. Ho DD, Neumann AU, Perelson AS, Chen W, et al.
Rapid turnover of plasma virions and CD4 lymphocytes in HIV-1 infection.
Nature 1995;373:123-126.
74. Holodniy M, Mole L, Winters M, Merigan TC. Diurnal
and short-term stability of HIV virus load as measured by gene
amplification. J. Acquir. Immun. Defic. Syndr. 1994;7: 363-8.
75. O. Brien W, Hartigan PM, Martin D, Esinhart J, et
al. Changes in plasma HIV-1 RNA and CD4+ lymphocyte counts and the risk
of progression to AIDS. Veterans Affairs Cooperative Study Group on
AIDS. NEJM 1996;334: 426-31.
76. Schapiro JM, Winters MA, Stewart F, Efron B, et al.
The effect of high-dose saquinavir on viral load and CD4+ T-cell counts
in HIV-infected patients. Ann. Int. Med. 1996;124:1039-50.
77. Francis DP. The search for the cause. In: Cahill
KM, ed. The AIDS epidemic. 1st ed. Melbourne: Hutchinson Publishing
Group, 1983: 137-150.
78. Guilbert B, Fellous M, Avrameas S. HLA-DR-specific
monoclonal antibodies cross-react with several self and nonself non-MHC
molecules. Immunogenetics 1986;24:118-121.
79. Gonzalez-Quintial R, Baccala R, Alzari PM, Nahmias
C, et al. Poly(Glu60Ala30Tyr10) (GAT)-induced IgG monclonal antibodies
cross-react with various self and non-self antigens through the
complentarity determining regions. Comparison with IgM monoclonal
polyreactive natural antibodies. Euro. J. Immunol.
1990;20:2383-2387.
80. Berzofsky JA, Berkower IJ, Epstein SL.
Antigen-Antibody Interactions and Monoclonal Antibodies. In: Paul WE,
ed. Fundamental Immunology. 3rd ed. New York: Raven, 1993:
421-465.
81. Fauci AS, Lane HC. Human Immunodeficiency Virus
(HIV) Disease: AIDS and Related Disorders. In: Isselbacher KJ, Braunwald
E, Wilson JD, Martin JB, Fauci AS, Kasper DL, ed. Harrison's Principles
of Internal Medicine. 13th ed. New York: McGraw-Hill Inc., 1994:
1566-1618.
82. Owen M, Steward M. Antigen recognition. In: Roitt
I, Brostoff J, Male D, ed. Immunology. 4th ed. London: Mosby, 1996:
7.1-7.12.
83. Ternynck T, Avrameas S. Murine natural monoclonal
antibodies: a study of their polyspecificities and their affinities.
Immunological Reviews 1986;94:99-112.
84. Pontes de Carvalho LC. The faitfullness of the
immunoglobulin molecule: can monoclonal antibodies ever be monospecific.
Immunol. Today 1986;7:33.
85. Chassagne J, Verelle P, Fonck Y, Legros M, et al.
Detection of lymphadenopathy-associated virus p18 in cells of patients
with lymphoid diseases using a monclonal antibody. Ann. Institut.
Past./Immunol. 1986;137D:403-408.
86. Parravicini CL, Klatzmann D, Jaffray P, Costanzi G,
et al. Monoclonal antibodies to the human immunodeficiency virus p18
protein cross-react with normal human tissues. AIDS
1988;2:171-177.
87. Guilbert B, Mahana W, Gilbert M, Mazie JC, et al.
Presence of natural autoantibodies in hyperimmunized mice. Immunol.
1985;56:401-8.
88. Matsiota P, Chamaret S, Montagnier L. Detection of
Natural Autoantibodies in the serum of Anti-HIV Positive-Individuals.
Ann. Institut. Past./Immunol. 1987;138:223-233.
89. Bess JW, Gorelick RJ, Bosche WJ, Henderson LE, et
al. Microvesicles are a source of contaminating cellular proteins found
in purified HIV-1 preparations. Virol. 1997;230:134-144.
90. Arthur LO, Bess JW, Jr., Urban RG, Strominger JL,
et al. Macaques immunized with HLA-DR are protected from challenge with
simian immunodeficiency virus. J. Virol. 1995;69:3117-24.
91. Sasaki H, Nakamura M, Ohno T, Matsuda Y, et al.
Myosin-actin interaction plays an important role in human
immunodeficiency virus type 1 release from target cells. Proc. Natl.
Acad. Sci. U S A 1995;92:2026-2030.
92. Pearce-Pratt R, Malamud D, Phillips DM. Role of
cytoskeleton in cell-to-cell transmission of human immunodeficiency
virus. J. Virol. 1994;68:2898-2905.
93. Choudhury S, El-Farrash MA, Kuroda MJ, Harada S.
Retention of HIV-1 inside infected MOLT-4 cells in association with
adhesion-induced cytoskeleton reorganisation. AIDS
1996;10:363-368.
94. Arthur LO, Bess JW, Sowder II RC, Beneviste RE, et
al. Cellular proteins bound to immunodeficiency viruses:implications for
pathogenesis and vaccines. Science 1992;258:1935-1938.
95. Chamaret S, Squinazi F, Courtois Y, Montagnier L.
Presence of anti-HIV antibodies in used syringes left out in public
places, beaches or collected through exchange programs. XIth
International Conference on AIDS. Vancouver:1996.
96. Modrow S, Hahn B, Shaw GM, Gallo RC, et al.
Computer-assisted analysis of envelope protein sequences of seven human
immunodeficiency virus isolates: prediction of antigenic epitopes in
conserved and variable regions. J. Virol. 1987;61:570-578.
97. Frank H. Oncovirinae: Type C Oncoviruses. In:
Nermut MV, Steven AC, ed. Animal Virus and Structure. Oxford: Elsevier,
1987: 273-287.